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目的:研究不同浓度盐酸小檗碱(Ber)单用及联合应用顺铂(DDP)对宫颈癌Hela细胞凋亡的影响,并探讨其抗癌机制。方法:将不同浓度的盐酸Ber、DDP分别或联合作用于宫颈癌Hela细胞,采用MTT比色法检测细胞体外增殖的抑制作用;原位末端标记法(TUNEL法)检测细胞凋亡率;免疫组化SABC法检测Hela细胞中NF-κBp65的表达。结果:MTT比色法表明盐酸Ber能抑制宫颈癌Hela细胞的体外增殖,并具有剂量、时间的依赖性(P<0.05);TUNEL法可检测到凋亡细胞,其凋亡率显示盐酸Ber诱导宫颈癌Hela细胞凋亡表现出剂量和时间的依赖性(P<0.05);免疫组化SABC法结果显示,盐酸Ber能引起细胞内NF-κBp65表达下调,与对照组相比差异有统计学意义(P<0.01);盐酸Ber与DDP联合用药表现出明显的协同作用,且高剂量的盐酸Ber可增加DDP的疗效。结论:盐酸Ber对宫颈癌Hela细胞体外增殖具有明显的抑制作用,且能增强DDP对宫颈癌Hela细胞的抑制作用,并增加其诱导宫颈癌Hela细胞的凋亡,其作用机制可能与降低NF-κBp65的表达有关。
Objective: To study the effects of berberine (Ber) alone and in combination with cisplatin (DDP) on the apoptosis of cervical cancer Hela cells and to explore its anticancer mechanism. Methods: Different concentrations of Ber and DDP hydrochloride were respectively applied to cervical cancer Hela cells. MTT colorimetric assay was used to detect the inhibition of proliferation in vitro. TUNEL method was used to detect the apoptosis rate. Immunohistochemistry The expression of NF-κBp65 in Hela cells was detected by SABC method. Results: MTT assay showed that berberine hydrochloride could inhibit the proliferation of Hela cells in vitro in a dose-and time-dependent manner (P <0.05). Apoptotic cells were detected by TUNEL assay and Ber induced by Berberine hydrochloride Cervical cancer Hela cells apoptosis showed dose-dependent and time-dependent (P <0.05); immunohistochemical SABC method results show that Berberine hydrochloride can cause intracellular expression of NF-κBp65 down, compared with the control group, the difference was statistically significant (P <0.01). The combination of Ber and DDP hydrochloride showed a synergistic effect, and the high dose of Ber could increase the efficacy of DDP. CONCLUSION: Berberine hydrochloride can significantly inhibit the proliferation of cervical cancer Hela cells in vitro and enhance the inhibitory effect of DDP on cervical cancer Hela cells and increase the apoptosis of Hela cells. The mechanism may be related to the decrease of NF- κBp65 expression.