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目的:唇腭裂是口腔颌面部最常见的先天性畸形,危害严重。mi RNA在细胞分化,生物发育及疾病发生发展过程中发挥巨大作用,越来越多的受到科研人员的关注。本课题对唇腭裂患儿下调表达的mi RNA与唇腭裂相关性进行验证研究,为mi RNA应用在唇腭裂防治中奠定一定的基础。方法:通过芯片分析方法检测唇腭裂患儿表达下调的mi RNA,利用MIRDB、TARGETSCAN-VERT和RNA22-HSA这三个生物信息学软件对唇腭裂患儿表达下调的mi RNA进行靶基因预测分析,验证候选mi RNA与唇腭裂具有相关性。结果:得出唇腭裂患儿中出现差异表达下调的mi RNA有hsa-mi R-3119,hsa-mi R-3915等73个,其中hsa-mi R-3611对应的靶基因GABRB3和hsa-mi R-764对应的靶基因F13A1有文献报道与唇腭裂具有相关性。结论:hsa-mi R-3611,hsa-mi R-764可能与唇腭裂的发生存在关联。
OBJECTIVE: Cleft lip and palate is the most common congenital malformation in oral and maxillofacial region, which is devastating. mi RNA plays a great role in the process of cell differentiation, biological development and disease development, and more and more researchers are concerned about it. This issue of cleft lip and palate patients with down-regulated expression of miRNA and cleft lip and palate related validation study for the miRNA application in the prevention and treatment of cleft lip and palate lay a solid foundation. Methods: The mi RNAs down-regulated in children with cleft lip and palate were detected by microarray analysis. Three bioinformatics softwares, MIRDB, TARGETSCAN-VERT and RNA22-HSA, were used to detect the mi RNAs that were down-regulated in children with cleft lip and palate. Verify candidate mi RNA is associated with cleft lip and palate. Results: There were 73 miRNAs that were differentially down-regulated in children with cleft lip and palate, such as hsa-mi R-3119 and hsa-mi R-3915, among which the target genes hsa-mi R- The target gene F13A1 corresponding to R-764 has been reported in the literature as having a correlation with cleft lip and palate. Conclusion: hsa-mi R-3611 and hsa-mi R-764 may be associated with cleft lip and palate.