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目的从线粒体角度观察参知健脑方组分对缺氧SH-SY5Y细胞的神经保护机制。方法将SH-SY5Y细胞分组并按照实验要求给予缺氧处理和参知健脑方组分干预。采用MTT法检测细胞存活率,采用大鼠8羟基脱氧鸟苷(8-OHd G)为标记物检测线粒体DNA损伤,采用ELISA法检测β-淀粉样蛋白前体蛋白(βAPP)和β-淀粉样多肽(Aβ)的含量。结果缺氧组细胞的死亡率、8-OHd G和Aβ的水平较正常组有明显升高(P<0.05)。而参知健脑方组经处理后,其水平比缺氧组有显著的减少。结论参知健脑方组分能有效的保护缺氧诱导的细胞死亡,改善线粒体DNA损伤,抑制毒性线粒体Aβ积累。提示了参知健脑方能通过线粒体途径对缺氧神经细胞发挥保护作用,为参知健脑方的临床应用提供实验支持。
Objective To observe the neuroprotective mechanism of Shenzhijiannao prescription on hypoxia SH-SY5Y cells from mitochondria. Methods SH-SY5Y cells were divided into groups and given hypoxic treatment and intervention of Shenzhi Jiannao Prescription according to the experimental requirements. Cell viability was determined by MTT assay. Mitochondrial DNA damage was detected by 8-OHdG (8-OHdG), and β-amyloid precursor protein (βAPP) and β-amyloid Polypeptide (Aβ) content. Results The mortality, the level of 8-OHd G and Aβ in hypoxia group were significantly higher than those in normal group (P <0.05). However, ShenzhiJianNanFang treated group showed a significant decrease compared with hypoxia group. Conclusion Shenzhijiannao Prescription can effectively protect hypoxia-induced cell death, improve mitochondrial DNA damage and inhibit the accumulation of toxic mitochondrial Aβ. It suggests that Shenzhi Jiannao can protect the hypoxic neurons through the mitochondrial pathway and provide experimental support for the clinical application of Shenzhi Jiannao Fang.