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研究氧化低密度脂蛋白（ｏｘＬＤＬ）对内皮细胞的损伤作用。方法：以胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入法和胆固醇酯薄层层析法，观察ｏｘＬＤＬ对培养的人脐静脉内皮细胞（ＨＵＶＥＣ）ＤＮＡ合成和胆固醇酯含量的影响。结果：ｏｘＬＤＬ抑制内皮细胞ＤＮＡ合成，并与浓度及作用时间相关，１００ｍｇ·Ｌ－１的ｏｘＬＤＬ作用２４ｈ的内皮细胞掺入３Ｈ－ＴｄＲ的ｄｐｍ数为不加ＬＤＬ组的４８％（Ｐ＜０．００１），天然ＬＤＬ（ｎＬＤＬ）对ＨＵＶＥＣ掺入３Ｈ－ＴｄＲ也有轻度的抑制作用。ｏｘＬＤＬ降低ＨＵＶＥＣ内胆固醇酯的含量，５０ｍｇ·Ｌ－１ｏｘＬＤＬ孵育的ＨＵＶＥＣ胞内胆固醇酯含量是ｎＬＤＬ组的４２％。扫描电镜发现ｏｘＬＤＬ孵育的ＨＵＶＥＣ形态发生改变，胞间连接减少。结论：ｏｘＬＤＬ对ＨＵＶＥＣ具有细胞毒作用，促发动脉粥样硬化。
To investigate the effect of oxLDL on endothelial cell injury. Methods: The effects of oxLDL on the DNA synthesis and cholesterol ester content of cultured human umbilical vein endothelial cells (HUVECs) were observed by incorporation of 3H-thymidine (3H-TdR) and cholesterol ester thin layer chromatography. Results: OxLDL inhibited the DNA synthesis of endothelial cells and was related to the concentration and duration of action. The number of dpm incorporation of 3H-TdR in endothelial cells treated with 100 mg · L-1 oxLDL for 48 h was 48% (P <0. 001). Natural LDL (nLDL) also had a mild inhibitory effect on HUVEC incorporation into 3H-TdR. OxLDL decreased the content of cholesteryl ester in HUVEC, and the intracellular cholesterol ester content in HUVEC incubated with 50 mg · L-1 oxLDL was 42% of nLDL group. Scanning electron microscopy showed that the morphology of HUVECs incubated with oxLDL changed and the number of intercellular junctions decreased. Conclusion: oxLDL has a cytotoxic effect on HUVEC and promotes atherosclerosis.