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目的 探讨可溶性Fas及其配体在小儿病毒性心肌炎中的表达以及与细胞凋亡和免疫反应的关系 ,旨在为病毒性心肌炎的诊断和治疗提供新的线索和依据。方法 采用ELISA法测定了 4 4例病毒性心肌炎患儿和 2 0例健康儿童血清中sFas和sFasL的表达水平 ,并进行前瞻性对比研究。结果 病毒性心肌炎患儿血清sFas和sFasL表达水平较健康儿童明显增高 (P <0 .0 1)。病毒性心肌炎患儿血清sFas与sFasL表达水平呈正相关性 (r=0 .6 5 0 9,P <0 .0 1)。儿童血清sFas和sFasL表达水平无性别差异 (P >0 .0 5 )。结论 ①血清sFas及sFasL表达增高与病毒性心肌炎疾病过程有关。②sFas表达增高可进一步证实病毒性心肌炎发病与免疫因素有关。③血清sFas表达增高可望作为T淋巴细胞激活的标志以及病毒性心肌炎诊断和治疗的重要依据。
Objective To investigate the expression of soluble Fas and its ligand in viral myocarditis and its relationship with apoptosis and immune responses in order to provide new clues and evidences for the diagnosis and treatment of viral myocarditis. Methods The serum levels of sFas and sFasL in 44 children with viral myocarditis and 20 healthy children were measured by ELISA and compared prospectively. Results The serum levels of sFas and sFasL in children with viral myocarditis were significantly higher than those in healthy children (P <0.01). The levels of sFas and sFasL in children with viral myocarditis were positively correlated (r = 0.609, P <0.01). Serum sFas and sFasL levels in children without gender differences (P> 0.05). Conclusion ① The increase of serum sFas and sFasL expression is related to the process of viral myocarditis. ② sFas increased expression may further confirm the pathogenesis of viral myocarditis and immune factors. ③ serum sFas increased expression is expected to be used as a marker of T lymphocyte activation and viral myocarditis diagnosis and treatment of important basis.