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目的通过姜黄素(Cur)影响中波紫外线(UVB)辐射HaCaT细胞线粒体膜电位(Δψ)及其相关因子,探讨其对UVB损伤细胞的保护作用及其机制。方法HaCaT细胞经UVB辐射后,加入不同剂量姜黄素继续培养,通过罗丹明123和Fluo-3/AM荧光强度检测细胞Δψ电位和细胞内游离钙离子浓度([Ca2+]i),以流式细胞术检测caspase-3和细胞色素c(Cytc)凋亡相关蛋白的表达。结果UVB辐射HaCaT细胞后,低线粒体膜电位的细胞数为(80.8±2.23)%,[Ca2+]i为(71.9±1.61)%,caspase-3和Cytc蛋白表达量分别为(29.00±1.60)%和(27.5±1.60)%,与对照组比较,差异均有统计学意义(P<0.01);加入姜黄素后与UVB组比较,细胞线粒体膜电位、[Ca2+]i及Cytc和caspase-3蛋白表达均呈剂量-效应关系,即随着药物浓度的增加,具有低细胞线粒体膜电位的细胞数、Cytc和caspase-3蛋白表达均显著减少,[Ca2+]i显著降低。结论姜黄素可能通过提高细胞线粒体膜电位,降低[Ca2+]i含量,减弱Cytc和caspase-3的级联反应,达到其抑制UVB辐射引起的HaCaT细胞凋亡,具有对UVB损伤HaCaT细胞的保护作用。
Objective To investigate the protective effect and mechanism of Cur (Cur) on UVB-induced mitochondrial membrane potential (Δψ) and its related factors in HaCaT cells. Methods After HaCaT cells were irradiated with UVB, different doses of curcumin were added and cultured. The Δψpotential and intracellular free calcium ion concentration ([Ca2+]i) of cells were detected by Rhodamine 123 and Fluo-3/AM fluorescence intensity. Caspase-3 and cytochrome c (Cytc) apoptosis-related protein expression was detected. Results After HaCaT cells were irradiated with UVB, the number of cells with low mitochondrial membrane potential was (80.8±2.23)%, [Ca2+]i was (71.9±1.61)%, and the expression levels of caspase-3 and Cytc protein were (29.00±1.60)%, respectively. And (27.5±1.60)%, compared with the control group, the difference was statistically significant (P<0.01); after adding curcumin compared with UVB group, the cell mitochondrial membrane potential, [Ca2+]i and Cytc and caspase-3 protein The expression showed a dose-effect relationship. With the increase of drug concentration, the number of cells with low cell mitochondrial membrane potential, the expression of Cytc and caspase-3 protein were significantly reduced, and [Ca2+]i was significantly decreased. Conclusion Curcumin may increase the mitochondrial membrane potential, decrease the content of [Ca 2+ ]i, weaken the cascade reaction of Cytc and caspase-3, and achieve the protective effect against UVB-induced HaCaT cell apoptosis in HaCaT cells. .