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目的：探讨FHIT基因与大肠癌的关系。方法：采用RTPCR及PCR产物直接测序法，对30例大肠癌组织及其配对正常组织、4个结肠癌细胞系的FHIT基因进行检测。结果：在8例(26.7％)大肠癌组织和2个（50％）结肠癌细胞系中检测到异常FHIT转录本，配对正常组织均无异常FHIT转录本；测序证实异常转录本缺失1～3个FHIT外显子。异常FHIT转录本与大肠癌患者的年龄、性别、血清CEA水平、肿瘤部位、大小、组织学类型、分化程度及病理分期均无关(P>0.100)。结论：在大肠癌发生中，异常FHIT转录本的表达是常见的事件；FHIT基因异常与大肠癌的发生发展有关。
Objective: To investigate the relationship between FHIT gene and colorectal cancer. METHODS: RT-PCR and direct sequencing of PCR products were used to detect FHIT gene in 30 colorectal cancer tissues, their matched normal tissues, and four colon cancer cell lines. RESULTS: Abnormal FHIT transcripts were detected in 8 (26.7%) colorectal cancer tissues and 2 (50%) colon cancer cell lines. There were no abnormal FHIT transcripts in paired normal tissues; sequencing confirmed the deletion of abnormal transcripts 1 to 3 FHIT exons. The abnormal FHIT transcript was not related to the age, sex, serum CEA level, tumor location, size, histological type, differentiation and pathological stage of patients with colorectal cancer (P>0.100). CONCLUSIONS: Abnormal expression of FHIT transcripts is a common event in the development of colorectal cancer. FHIT abnormalities are associated with the development of colorectal cancer.