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[目的]探讨纳米金属材料应用于红掌遗传转化中抑制农杆菌污染的新技术。[方法]分别在农杆菌培养基和红掌愈伤组织培养基中添加不同种类的纳米金属材料,调查其对农杆菌生长、愈伤组织生长及分化、农杆菌污染抑制效果的影响。[结果]在供试的四种纳米金属材料中,纳米载银试剂对农杆菌生长有显著的抑制作用,其最低抑菌浓度为25 mg/L;在红掌转基因材料受到农杆菌污染的情况下,采用25 mg/L的纳米载银试剂处理1 d,抑菌效果良好,抑菌率可达100%,并且对红掌愈伤组织生长及再分化无不良影响。[结论]纳米载银试剂能够有效抑制农杆菌污染,其抑菌效果显著优于头孢霉素和羧苄青霉素。
[Objective] The research aimed to discuss the new technology of inhibiting the Agrobacterium contamination in the genetic transformation of Anthurium by nanomaterials. [Method] Different kinds of nano-metal materials were added to Agrobacterium culture medium and Anthurium calli callus respectively to investigate their effects on Agrobacterium growth, callus growth and differentiation, and Agrobacterium tumefaciens pollution inhibition. [Result] Among the four kinds of nano-metal materials tested, nano silver-loading reagent could significantly inhibit the growth of Agrobacterium with the minimum inhibitory concentration of 25 mg / L. When the rhizoma mahogany was contaminated by Agrobacterium Under the condition of 25 mg / L nano-silver-loaded reagent for 1 d, the antibacterial effect was good, the antibacterial rate was up to 100%, and had no adverse effect on the growth and re-differentiation of callus. [Conclusion] The silver-loaded silver reagent can effectively inhibit the Agrobacterium tumefaciens pollution and its antibacterial effect is significantly better than that of cefotaxime and carbenicillin.