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目的 :研究金属硫蛋白 (MT)在人正常结肠组织和结肠癌组织中的表达及其生物学意义。方法 :应用免疫组织化学方法检测 1 0例人正常结肠组织和 2 0例结肠癌组织标本中金属硫蛋白的表达水平 ,比较MT表达的差别。结果 :MT在人正常结肠组织和结肠癌组织中的阳性率均为 1 0 0 % ,用HPISA - 1 0 0 0图像分析系统测得其不同的平均阳性灰度值 (AGA)分别为 6 7 5 4± 7 4 6 ,93 71± 9 1 2。MT在人正常结肠组织和结肠癌组织中的表达有显著性差异 (p <0 0 5 )。结论 :MT在人正常结肠组织和结肠癌组织中的不同表达显示MT与结肠癌关系密切 ,对结肠组织的恶性变具有一定的意义
Objective: To study the expression and biological significance of metallothionein (MT) in human normal colon and colon cancer tissues. Methods: Immunohistochemistry was used to detect the expression of metallothionein in 10 normal human colon tissues and 20 colon cancer tissues. The differences of MT expression were compared. RESULTS: The positive rates of MT in human normal colorectal cancer and colon cancer tissues were 100%. The different mean positive grayscale values (AGA) of the MT measured by the HPISA - 1000 image analysis system were 67 5 4 ± 7 4 6, 93 71 ± 9 1 2. The expression of MT in human normal colon and colon cancer tissues was significantly different (p <0.05). Conclusion: The different expression of MT in normal human colon and colon cancer tissues shows that MT is closely related to colon cancer and has certain significance to the malignant transformation of colon tissue