论文部分内容阅读
本研究探讨联合应用藤黄酸(GA)和磁性纳米Fe3O4颗粒(MNP-Fe3O4)治疗白血病的潜在可能性。采用MTT法检测GA对U937的细胞毒作用及GA联合MNP-Fe3O4对U937细胞生长抑制的影响;流式细胞术检测细胞凋亡率;应用Wright及DAPI染色观察凋亡细胞的形态学变化;采用实时定量RT-PCR及Western blot法分别检测细胞的caspase-3、bcl-2、bax、NF-κB和survivin基因及相应蛋白的表达。结果表明:GA对U937细胞的生长抑制作用呈现时间及剂量依赖性;MNP-Fe3O4本身无细胞毒作用,但能使GA对U937细胞的生长抑制作用增强;联合应用GA和MNP-Fe3O4诱导的细胞凋亡率较单用GA明显增加,细胞呈现典型的凋亡形态改变,同时可见caspase-3及bax表达上调,而凋亡抑制基因如bcl-2、NF-κB和survivin表达下调。结论:MNP-Fe3O4能增强GA对U937细胞的凋亡诱导作用,两者联合应用可能是一种新型而安全有效的白血病治疗方法。
This study explored the potential of combined use of gambogic acid (GA) and magnetic nano-Fe3O4 particles (MNP-Fe3O4) in the treatment of leukemia. The effects of GA on the cytotoxicity of U937 and the effect of GA combined with MNP-Fe3O4 on the growth of U937 cells were detected by MTT assay. The apoptosis rate was detected by flow cytometry. The morphological changes of apoptotic cells were observed by Wright and DAPI staining. The expression of caspase-3, bcl-2, bax, NF-κB and survivin genes and their corresponding proteins were detected by real-time RT-PCR and Western blot respectively. The results showed that: GA inhibited the growth of U937 cells in a time and dose-dependent manner; MNP-Fe3O4 itself had no cytotoxic effect, but enhanced the inhibitory effect of GA on U937 cells. Combined with GA and MNP-Fe3O4-induced cells The apoptotic rate was significantly higher than that of GA alone. The cells showed the typical morphological changes of apoptosis. At the same time, the expression of caspase-3 and bax was up-regulated while the expression of apoptosis-suppressing genes such as bcl-2, NF-κB and survivin were down-regulated. CONCLUSION: MNP-Fe3O4 can enhance the apoptosis-inducing effect of GA on U937 cells. The combination of the two may be a new and safe and effective treatment for leukemia.