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采用简单且方便的超声方法合成了具有叶酸受体(FR)靶向性的γ-环糊精-叶酸包合物(γ-CD/FA)修饰的CdSe/ZnS量子点(QDs)纳米粒。利用傅里叶红外光谱(FTIR)、核磁共振氢谱(1H NMR)、紫外可见吸收光谱(UV-vis)、荧光光谱和透射电子显微镜(TEM)等手段对其进行了表征。结果表明,FR靶向性的γ-CD/FA修饰的CdSe/ZnS QDs,不但具有较好的单分散性和较小的尺寸,还具有较高的量子产率(QY)和较长的荧光寿命等较好的光学性能。细胞毒性实验表明,FR靶向性的γ-CD/FA修饰的CdSe/ZnS QDs具有较低的毒性,还具有选择性地进入FR高表达的癌细胞内的功能。这种直径为4～5 nm的QDs比较容易进入细胞,在体内易被肾脏清除,比较适合于在生物探针或生物成像等生物医学方面的应用。
CdSe / ZnS quantum dots (QDs) nanoparticles modified with γ-cyclodextrin-folic acid inclusion complex (γ-CD / FA) with folate receptor (FR) targeting were synthesized by a simple and convenient sonication method. Their structures were characterized by FTIR, 1H NMR, UV-vis, fluorescence and TEM. The results showed that FR-targeted γ-CD / FA modified CdSe / ZnS QDs not only had better monodispersity and smaller size, but also had higher quantum yield (QY) and longer fluorescence Life and other better optical performance. Cytotoxicity experiments showed that FR-targeted γ-CD / FA-modified CdSe / ZnS QDs have lower toxicity and selectively enter the function of cancer cells with high FR expression. The QDs, 4 to 5 nm in diameter, are relatively easy to enter cells and are easily cleared by the kidneys in vivo, making them suitable for biomedical applications such as bioprobe or bioimaging.