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目的:观察褪黑激素(Mel)对老年小鼠大脑皮层突触体谷氨酸(Glu)释放以及KCl,Glu在原代培养胎鼠脑细胞诱发的神经毒性的影响,以探讨Mel抗衰老的作用机制。方法:制备老年小鼠大脑皮层突触体,用RF-5000型双波长荧光分光光度计检测谷氨酸释放量。应用原代培养的大鼠皮层细胞MTT染色和乳酸脱氢酶(LDH)测定法评估神经元活性。结果:Mel能够抑制高浓度氯化钾(30mmol·L~(-1))诱发的老年小鼠大脑皮层突触体钙依赖性及非依赖性谷氨酸释放,抵抗KCl和Glu诱发的皮层细胞损伤,对神经元有保护作用。结论:Mel对大脑皮层突触体谷氨酸释放的抑制作用以及对大脑皮层神经元的保护作用可能是其抗衰老作用机制之一。
Objective: To observe the effect of melatonin (Mel) on the release of glutamate (Glu) in brain cortex of aged mice and the neurotoxicity induced by KCl and Glu in primary cultured fetal rat brain cells to explore the anti-aging effect of Mel mechanism. Methods: Cerebral cortex synaptosomes were prepared from aged mice. The release of glutamate was measured by RF-5000 double-wavelength fluorescence spectrophotometer. Neuronal activity was assessed using primary cultured rat cortical MTT staining and lactate dehydrogenase (LDH) assay. Results: Mel inhibited the calcium-dependent and non-glutamate release of synaptosomes in cortex of aged mice induced by high concentration of potassium chloride (30 mmol·L -1), and inhibited the growth of cortical cells induced by KCl and Glu Injury, a protective effect on neurons. CONCLUSION: Mel inhibits the glutamate release of cerebral cortex synapse and protects the neurons of the cerebral cortex, which may be one of its anti-aging mechanisms.