I_3~-在紫外区有明显吸收,而维生素C分子结构中的烯二醇基的还原性可以定量地使其吸收减弱。据此本文提出了应用分光光度法标定VC标准溶液的含量。该法与经典的容量标定法相比,具有准确、快速、省料等优点。 I_3 ~ - in the UV absorption significantly, while the vitamin C molecular structure of the enediol-based reduction can quantitatively make its absorption weakened. Accordingly, this paper presents the application of spectrophotometric calibration of the content of VC standard solution. Compared with the classical volumetric calibration method, this method has the advantages of accuracy, rapidity, material saving and so on.