发酵豆渣工艺的优化及对营养成分的影响

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旨在以植物乳杆菌、枯草芽孢杆菌和酿酒酵母混合菌固态发酵豆渣饲料.利用正交试验根据混菌比例、接种量、水分、发酵温度和发酵时间等因素,对发酵后豆渣饲料的pH值、活菌数、还原糖、氨态氮浓度、乳酸和挥发性脂肪酸含量等指标的影响,筛选出混菌固态发酵豆渣饲料的最优工艺,并在此基础上,对发酵前后豆渣饲料的营养物质含量进行测定.结果 表明,混菌发酵豆渣饲料的最佳工艺条件为:3株试验菌的接种比例为:植物乳杆菌∶枯草芽孢杆菌∶酿酒酵母=3∶2∶1、接种量为10%、固态培养基含水量为50%、温度为35℃、发酵时间为48 h.与发酵前相比,发酵后豆渣饲料中的粗蛋白质、酸溶蛋白、粗灰分的含量显著提高(P<0.05),pH值显著下降(P<0.05).综上,以植物乳杆菌、枯草芽孢杆菌和酿酒酵母混合菌发酵豆渣饲料后,豆渣饲料的pH值降低且营养成分得到改善.
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