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目的观察双氢青蒿素(DHA)对前列腺癌PC-3细胞生长及Survivin表达的影响,并探讨其作用机制。方法PC-3细胞经不同浓度的DHA处理后,MTT法检测细胞增殖抑制率;将PC-3细胞注入裸鼠右侧腋窝下,建立裸鼠种植瘤模型,将20只模型鼠随机分为空白对照组、DMSO对照组、DHA高剂量组(200μmol/kg体重)和低剂量组(100μmol/kg体重),经13d干预后,计算抑瘤率;光镜及电镜观察肿瘤组织形态学改变,免疫组织化学法检测Survivin的表达,TUNEL法检测细胞凋亡。结果DHA可显著抑制PC-3细胞的增殖,并具有时间、浓度依赖性;DHA对裸鼠种植瘤生长具有明显的抑制作用,抑瘤率达60%以上;光镜及电镜观察到DHA两剂量组肿瘤组织中散在凋亡细胞及凋亡小体;DHA两剂量组细胞Survivin表达减弱,凋亡率明显升高。结论DHA可明显抑制PC-3细胞生长,其机制与下调Survivin的表达并诱导肿瘤细胞凋亡有关。
Objective To observe the effect of dihydroartemisinin (DHA) on the growth of prostate cancer PC-3 cells and the expression of Survivin, and to explore its mechanism. Methods PC-3 cells were treated with different concentrations of DHA. MTT assay was used to detect the cell proliferation inhibition rate. PC-3 cells were injected into the right armpit of nude mice to establish a model of implanted tumor in nude mice. Twenty models were randomly divided into blank The control group, DMSO control group, DHA high dose group (200μmol / kg body weight) and low dose group (100μmol / kg body weight), after 13d intervention, the inhibition rate of tumor was calculated. The morphological changes of tumor tissue were observed by light microscope and electron microscope Survivin expression was detected by histochemical method and apoptosis was detected by TUNEL method. Results DHA could significantly inhibit the proliferation of PC-3 cells in a time-and dose-dependent manner. DHA had a significant inhibitory effect on the growth of implanted tumors in nude mice, with a tumor inhibition rate of over 60%. DHA and two doses of DHA The apoptotic cells and apoptotic bodies were scattered in the tumor tissue. The expression of Survivin in the two doses of DHA group was weakened and the apoptosis rate was significantly increased. Conclusion DHA can significantly inhibit the growth of PC-3 cells, and its mechanism is related to down-regulating the expression of Survivin and inducing the apoptosis of tumor cells.