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目的研究肝细胞生长因子受体(c-Met)在喉鳞状细胞癌患者组织及血浆中的表达,探讨c-Met在喉鳞状细胞癌发生、发展所起的作用。方法喉鳞状细胞癌及喉乳头状瘤患者进行调查,并且设立正常对照组,应用酶联免疫吸附法检测各组血浆c-Met水平;应用半定量RT-PCR法和免疫组织化学法分别检测各组组织中c-Met mRNA及c-Met蛋白的表达。结果喉鳞状细胞癌患者血浆中的c-Met水平明显高于喉乳头状瘤患者和正常人群(均P<0.05)。喉鳞状细胞癌组织中的c-Met水平明显高于喉乳头状瘤组织和正常组织(均P<0.05)。结论 c-Met在喉鳞状细胞癌组织及血浆中呈高表达,可能与喉鳞状细胞癌的发生、发展有关。
Objective To investigate the expression of hepatocyte growth factor receptor (c-Met) in the tissues and plasma of patients with laryngeal squamous cell carcinoma and to explore the role of c-Met in the occurrence and development of laryngeal squamous cell carcinoma. Methods Laryngeal squamous cell carcinoma and laryngeal papilloma patients were investigated, and the normal control group was set up, the plasma c-Met levels were detected by enzyme-linked immunosorbent assay; semi-quantitative RT-PCR and immunohistochemistry were used to detect The expression of c-Met mRNA and c-Met protein in each group. Results The plasma level of c-Met in patients with laryngeal squamous cell carcinoma was significantly higher than that in patients with laryngeal papilloma and normal (P <0.05). The level of c-Met in laryngeal squamous cell carcinoma was significantly higher than that in laryngeal papilloma and normal tissues (both P <0.05). Conclusions c-Met is highly expressed in the tissues and plasma of laryngeal squamous cell carcinoma, which may be related to the occurrence and development of laryngeal squamous cell carcinoma.