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观察激素替代治疗时大鼠动脉雌激素受体 (ER)的表达及一氧化氮合酶 (NOS)的活性。方法 建立大鼠雌激素替代治疗模型 ,用放射配体结合分析法检测主动脉ER的含量 ,血红蛋白还原酶法检测主动脉中NOS的活性。结果 卵巢切除去势组大鼠动脉ER的结合容量及NOS活性显著低于假手术组 ;切除卵巢后补充雌激素鼠动脉ER的结合容量及NOS活性都显著高于单纯卵巢切除去势组。结论 雌性大鼠去势 2个月后 ,主动脉表达ER显著减少 ,NOS活性显著减低 ,补充适量雌激素能使去势大鼠动脉NOS的活性显著增强 ,ER的含量维持在正常水平。这可能是雌激素对绝经后妇女心血管保护作用的重要机制。
To observe the expression of estrogen receptor (ER) and the activity of nitric oxide synthase (NOS) in rats during hormone replacement therapy. Methods The rat model of estrogen replacement therapy was established. The content of aortic ER was detected by radioligand binding assay and the activity of NOS in the aorta was detected by hemoglobin reductase. RESULTS: The ER binding capacity and NOS activity in arteries of ovariectomized ovariectomized rats were significantly lower than that of sham-operated rats. The ER binding capacity and NOS activity of estrogen-fed mice after ovariectomy were significantly higher than those of ovariectomized ovariectomized rats. Conclusion After 2 months of ovariectomy in female rats, ER expression in the aorta was significantly decreased and NOS activity was significantly reduced. Supplementation of appropriate estrogen could significantly increase the activity of NOS in arteries of ovariectomized rats and maintain the normal level of ER. This may be an important mechanism of cardiovascular protection of estrogen in postmenopausal women.