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静态法涂制毛细管色谱柱中的封口是至关重要的一个步骤。将毛细管末端插入硅橡胶封口,方法简便、快速、效果好。取5mm厚硅橡胶一片,切取长20mm以上,宽5～10mm不等的一小块,在侧向上先用注射针头(直径依毛细管外径而定,对弹性石英毛细管,取用4号针头)扎—10mm长的小孔。待固定液填充完毕后,将氮气压力表关闭。毛细管两端压力平衡后,用手握住末端约10圈左右,末端有固定液溶液溢出,立即将其插入硅橡胶片的小孔中12～15mm,取下毛细管柱,整个封口即成。
The static method of sealing the capillaries in a capillary column is a crucial step. The end of the capillary into the silicone rubber seal, the method is simple, fast, effective. Take a 5mm thick silicone rubber, cut a length of 20mm or more, 5 ~ 10mm wide ranging from the first injection needle with a lateral (diameter according to the capillary diameter may be, for flexible quartz capillary, access to 4 needles) Tie-10mm long hole. After the fixed liquid is filled, close the nitrogen manometer. Capillary pressure balance at both ends, the hand holding the end of about 10 laps, the end of a fixed solution overflow, immediately insert the hole in the silicone rubber sheet 12 ~ 15mm, remove the capillary column, the entire seal Serve.