应用本室构建的龋齿疫苗工程菌株及 GTF抗原免疫母鸡 ,取鸡卵黄以水 -硫酸铵 -离子交换层析法提取纯化蛋黄抗体 (Ig Y ) ,以放免法测定血清及蛋黄中的抗体。结果用上述 2种抗原免疫的鸡 ,在血清及蛋黄中均能检出抗 GTF抗体。提纯的蛋黄抗体 ,经 SDS- PAGE电泳检测 ,相对分子质量约为 2 2 0 0 0 0 ,纯度达到 97.9% ,经 2 -巯基乙醇处理后 ,分为重链和轻链 ,相对分子质量分别约为 76 0 0 0和 2 80 0 0。经免疫印迹分析 ,具有良好的免疫学特异性。 The caries vaccine strain and the GTF antigen-immunized hens were used in this study. IgY was extracted from chicken egg yolk by water-ammonium sulfate-ion exchange chromatography and the antibodies in serum and egg yolk were determined by radioimmunoassay. Results The chickens immunized with the above two antigens showed anti-GTF antibodies in both serum and egg yolk. The purified yolk antibody was detected by SDS-PAGE. The relative molecular mass was about 22,000 and the purity was 97.9%. After being treated with 2-mercaptoethanol, the antibody was divided into heavy chain and light chain, and the relative molecular masses were about 76 0 0 0 and 2 80 0 0. After immunoblot analysis, has good immunological specificity.