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[目的]观察自然衰老骨质疏松模型中雌性大鼠骨钙含量、骨密度、雌激素及雌激素受表达、维生素D受体表达改变,探索老年妇女骨质疏松症发生发展的机制,以寻求更有效的治疗途径。[方法]选择健康纯种雌性SD大鼠12只,喂养22月后复制自然衰老骨质疏松模型,另随机选择6月龄雌性大鼠10只为青年对照组,观察各组实验大鼠骨钙含量、骨密度、雌激素及雌激素受表达、维生素D受体表达。[结果]①老年模型组(81.16±16.88)mg/ml骨钙含量较青年对照组(152.59±21.96)mg/ml显著降低(46.6%,P﹤0.05);②老年模型组股骨骨密度(64.3±8.1)g/cm2较青年对照组(81.8±7.2)g/cm2显著降低(21.4%,P﹤0.05);③老年模型组血清雌激素(8.60±3.79)ng/ml及骨组织雌激素受体(124.6±3.3)较青年对照组(17.64±17.53)ng/ml,(96.3±2.5)显著降低(51.1%,22.7%,P﹤0.05),维生素D受体(145.6±3.0)比(156.2±1.3)降低,但尚未形成统计学差异。[结论]老年雌性大鼠中骨钙含量、骨密度、雌激素及雌激素受体发生显著性改变,提示可作为老年骨质疏松研究的模型之一,来探求骨质疏松的发病机理和治疗途径。
[Objective] To observe the changes of bone calcium, bone mineral density, estrogen and estrogen expression and vitamin D receptor expression in female rats with natural aging osteoporosis and to explore the mechanism of osteoporosis in elderly women. More effective treatment. [Methods] Twelve healthy SD female SD rats were selected and fed with 22-month old model of natural aging osteoporosis. Another 10 female 6-month-old female rats were randomly selected as young control group. The bone calcium Content, bone mineral density, estrogen and estrogen expression, vitamin D receptor expression. [Results] ① The bone calcium content in aged model group (81.16 ± 16.88) mg / ml was significantly lower than that in young control group (152.59 ± 21.96) mg / ml (46.6%, P <0.05) ± 8.1) g / cm2 was significantly lower than that of the young control group (81.8 ± 7.2) g / cm2 (21.4%, P <0.05); ③The serum estrogen (8.60 ± 3.79) ng / (124.6 ± 3.3) was significantly lower than that of the youth control group (17.64 ± 17.53) ng / ml, (96.3 ± 2.5) (51.1% vs 22.7%, P <0.05) ± 1.3), but no statistical difference has been found. [Conclusion] The changes of bone calcium, bone mineral density, estrogen and estrogen receptor in aged female rats have significant changes, suggesting that it may be used as one of the models for senile osteoporosis to explore the pathogenesis and treatment of osteoporosis way.