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目的探讨过氧化物酶体增殖因子激活受体α激动剂非诺贝特诱导雄激素非依赖性前列腺癌DU145细胞氧化损伤和凋亡的作用及可能机制。方法用0、50、100μmol/L浓度的非诺贝特作用于DU145细胞,24h后利用荧光显微镜和流式细胞仪检测各组细胞中活性氧、超氧化物阴离子、一氧化氮含量和细胞凋亡率的变化。Westernblot检测细胞内凋亡相关蛋白BCL-2和BAX的表达变化。结果不同浓度非诺贝特作用细胞后细胞凋亡率显著上调,活性氧、超氧化物阴离子和一氧化氮含量较对照组明显上升并呈剂量依赖性(P<0.05)。50μmol/L非诺贝特作用后,DU145细胞内BCL-2表达明显下调,BAX表达升高。结论非诺贝特能诱导前列腺癌细胞DU145氧化损伤和细胞凋亡,其机制可能与氧化应激增强及抗凋亡蛋白表达下降有关。
Objective To investigate the effects and mechanisms of peroxisome proliferator-activated receptor α agonist fenofibrate on oxidative damage and apoptosis of androgen-independent prostate cancer DU145 cells. Methods DU145 cells were treated with fenofibrate at concentrations of 0, 50 and 100 μmol / L. After 24h, the contents of ROS, superoxide anion, nitric oxide and apoptosis in the cells were detected by fluorescence microscopy and flow cytometry Mortality changes. Western blot was used to detect the expression of apoptosis-related proteins BCL-2 and BAX. Results The apoptotic rates of fenofibrate-treated cells were significantly up-regulated and the levels of ROS, superoxide anion and nitric oxide were significantly increased compared with the control group in a dose-dependent manner (P <0.05). After 50 μmol / L fenofibrate, the expression of BCL-2 in DU145 cells was significantly down-regulated and the expression of BAX was increased. Conclusion Fenofibrate can induce the oxidative damage and apoptosis of DU145 prostate cancer cells, which may be related to the increase of oxidative stress and the decrease of anti-apoptotic protein expression.