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目的:通过了解宣白承气汤与其拆方对内毒素诱导的急性肺损伤(ALI)大鼠肺和大肠组织中P物质的影响,从神经肽方面去研究ALI“从肠论治”的有效机理。方法:35只雄性Wistar大鼠随机分为对照组、治肺组、治肠组、肺肠同治组、模型组。采取气管内注入脂多糖的方法制备ALI大鼠模型。各治疗组在造模前相应药物灌胃6 d,而对照组和模型组在相应时间点予等容积生理盐水灌胃,第6天灌胃后0.5 h造模,造模4 h后测肺组织湿/干重比(W/D),用ELISA法检测肺、大肠组织中P物质的含量,并观察肺组织病理变化。结果:与对照组相比,模型组大鼠肺组织的W/D升高(P<0.05),模型组大鼠肺组织P物质含量明显升高,肠组织里的P物质显著降低(P<0.01);对比模型组,治肠组、肺肠同治组W/D下降(P<0.05),治肺组、治肠组、肺肠同治组肺组织中的P物质含量都显著降低(P<0.01),肠组织中的P物质都显著升高(P<0.01或P<0.05);对比治肺组,肺肠同治组肺组织中P物质显著降低(P<0.01),而肠组织中P物质却升高(P<0.05);光镜下模型组呈现ALI的病理改变,而各治疗组较模型组均有改善,肺肠同治组改善较明显。结论:通利大肠或在治肺的同时增进通利大肠,会改变ALI大鼠肺及大肠组织中P物质含量,这有可能是“从肠论治”ALI有效的机理之一。
OBJECTIVE: To understand the effect of XuanBaiChengqi Decoction and its decomposed formulas on substance P in the lung and colorectal tissue of rats with acute lung injury induced by endotoxin (ALI) Effective mechanism. Methods: Thirty-five male Wistar rats were randomly divided into control group, treatment of lung group, treatment of intestine group, intestine of lung intestine group and model group. ALI rat model was prepared by intratracheal injection of lipopolysaccharide. Before treatment, the rats in each treatment group were given gavage for 6 days, while the control group and model group were intragastrically given normal saline at the corresponding time points, 0.5 h after gavage on day 6, The wet / dry weight ratio (W / D) was measured. The contents of substance P in the lung and colorectal tissues were detected by ELISA, and the pathological changes of the lung tissue were observed. Results: Compared with the control group, the W / D of lung tissue in the model group was significantly increased (P <0.05), and the content of substance P in the lung tissue of the model group was significantly increased and the substance P in the intestinal tissue was significantly decreased (P < 0.01). Compared with model group, the W / D decreased (P <0.05), and the content of substance P in the lungs of the treatment group, the treatment group and the lung intestine group were significantly decreased (P < 0.01). The content of substance P in intestinal tissue was significantly increased (P <0.01 or P <0.05). Compared with control group, the content of substance P in lung tissue of lung and intestine was significantly decreased (P <0.01) (P <0.05). The pathological changes of ALI were observed in the model group under light microscope, while those in the treatment group were better than those in the model group. Conclusion: Tongli’s large intestine or Tongli’s intestine can improve the content of substance P in the lung and colorectal tissues of ALI rats, which may be one of the effective mechanisms of ALI.