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Two-dimensional material has been widely investi-gated for potential applications in sensor and flexible electronics. In this work, a self-powered flexible humidity sensing device based on poly(vinyl alcohol)/Ti3C2Tx (PVA/MXene) nanofibers film and mon-olayer molybdenum diselenide (MoSe2) piezoelectric nanogen-erator (PENG) was reported for the first time. The monolayer MoSe2-based PENG was fabri- cated by atmospheric pressure chemical vapor deposition techniques, which can generate a peak output of 35 mV and a power density of 42 mW m?2. The flexible PENG integrated on polyethylene terephthalate (PET) substrate can harvest energy generated by different parts of human body and exhibit great application prospects in wearable devices. The electrospinned PVA/MXene nanofiber-based humidity sensor with flexible PET substrate under the driven of monolayer MoSe2 PENG, shows high response of ～40, fast response/recovery time of 0.9/6.3 s, low hysteresis of 1.8％ and excellent repeatability. The self-powered flexible humidity sensor yields the capability of detecting human skin moisture and ambient humidity. This work provides a pathway to explore the high-performance humidity sensor integrated with PENG for the self-powered flexible electronic devices.