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利用国家果树种质南京桃资源圃中保存的 630 份资源的 60 个描述项信息,通过比较分组、逐步聚类和随机 3 类取样方法,以及取样比例的筛选,进行了初级核心种质构建研究。结果显示:逐步聚类取样方法优于分组取样和完全随机取样方法;逐步聚类取样方法的最适取样比例为 15%;不同聚类模型间存在差异,聚类后的取样方法间也存在差异;欧式距离和内平方距离法逐步聚类后优先选取高均偏种质的方法更适宜桃初级核心种质的构建。通过缺失表型添加,构建的初级核心种质包括 99 份资源,占原始库的 15.7%,具 100%表型保留比例。经多样性指数、表型方差、变异系数检验,以及数量性状的均值和极差符合率检验,初级核心种质具有良好的代表性。
Based on the information of 60 descriptive items of 630 resources preserved in the national fruit tree germplasm resources in Nanjing Peach Garden, preliminary core collection studies were conducted by comparative grouping, stepwise clustering and random sampling of 3 types of samples, . The results showed that stepwise cluster sampling method was superior to group sampling method and completely random sampling method. The optimal sampling proportion of stepwise clustering sampling method was 15%. There were differences among different cluster models. There was also difference between clustering sampling methods The method of preferentially selecting high average-deviation germplasm after the gradual clustering based on the Euclidean distance and the inner squared distance method is more suitable for the construction of the peach primary core germplasm. By deletion phenotype addition, the constructed primary core collection comprised 99 resources, representing 15.7% of the original pool, with a 100% phenotypic retention ratio. By the diversity index, phenotypic variance, coefficient of variation test, as well as the quantitative traits of the mean and poor coincidence test, the primary core collection has a good representation.