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大肠杆菌是最广泛用于异源性蛋白合成的原核系统.最适表达系统一旦构建成功,通过增加每单位时间每个菌体的蛋白产量(单位生产率),或通过增加每单位时间的菌体浓度(菌体生产率)而使蛋白产量增加.已开发出在加料分批培养中培养重组与非重组大肠杆菌株达每升100g(菌体干重)以上收量的各种高细胞密度培养(HCDC)技术.本文综述了大肠杆菌HCDC所遇到的问题,讨论了不同的解决办法.还介绍了大肠杆菌HCDC的加料策略及使用这些策略所得到的结果.
Escherichia coli is the most widely used prokaryotic system for heterologous protein synthesis.Once the optimal expression system is constructed successfully, by increasing the protein production (per unit of production) per cell per unit of time, or by increasing the number of cells per unit of time Concentration (cell productivity) and increased protein yield Various high cell density cultures (above dry weight of cells) of up to 100 g per liter of recombinant and non-recombinant E. coli strains cultured in fed-batch culture have been developed HCDC) technology.This paper reviews the problems encountered in E. coli HCDC, discusses different solutions, and also introduces the feeding strategy of E. coli HCDC and the results obtained using these strategies.