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作者对31例再生障碍性贫血患者进行了淋巴细胞亚群及淋巴细胞功能测定。31例中14例为重型再障。男15例,女16例,平均年龄39±19.2岁。11例在初次诊断时进行检查,20例在诊断后不同病期进行检查,以年龄及性别相当的100例正常人作对照组。测定方法:取周围血,用 Ficoll-Hypague 分离得单个核细胞(MNC),调细胞浓度为1×10~6/ml。作以下检查。淋巴细胞亚群:用纯化的单克隆抗体 Leu1.Leu2.Leu3.OKIa1,以间接萤光法测定。对商陆丝裂原的反应性:取细胞悬液0.2ml 加入商陆丝裂原,使商陆最终浓度为0.1ml,经37℃、
The author of 31 cases of aplastic anemia in patients with lymphocyte subsets and lymphocyte function determination. Fourteen of 31 cases were aplastic aneurysms. There were 15 males and 16 females, with an average age of 39 ± 19.2 years. Eleven cases were examined at initial diagnosis, 20 cases were examined at different stages after diagnosis, and 100 normal subjects of the same age and gender as control group. Determination: Take peripheral blood, mononuclear cells (MNC) were isolated by Ficoll-Hypague, adjusted cell concentration of 1 × 10 ~ 6 / ml. Make the following check. Lymphocyte subpopulations: The purified monoclonal antibody Leu1.Leu2.Leu3.OKIa1 was measured by indirect fluorescence. The reactivity of Phyllostachys praeparatum: Take 0.2ml of cell suspension was added to Phytolacca, the final concentration of Phragmites aurantium 0.1ml, after 37 ℃,