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用16个玉米自交系同C型、Y型和YⅡ型雄性不育系测交、回交,以了解不育系的恢保关系;将C型不育系测交F1中的可育株自交,同时与C型不育系回交。以分析C型不育系恢复性的遗传行为。结果表明,5003、330、白330、035-6、木6-11等5个自交系为C型不育系的恢复系,32-2、贞367、i-1等3个自交系保持C型不育系的不育性;TR3702、77-1、承10-9、5003、449、交51等6个自交系保持Y型不育系的不育性;贞367、107-1为YⅡ型不育系的恢复系,TR3702、青C2、035-6、330、5003等5个自交系保持YⅡ型不育系的不育性。32-2对C型、承10-9对Y型、5003对Y型和YⅡ型的回交F1全部不育,可用连续回交法转育成新不育系。C型不育系测交F2出现可育与不育的3∶1育性分离,回交一代出现1∶1的育性分离,表明恢复性受一对显性基因控制。
16 maize inbred lines with C type, Y type and Y Ⅱ type male sterile line measured cross, backcrossed to understand the relationship between male and female lines of CMS; F1 CMS sterile line measured cross in F1 Selfing, at the same time backcrossed with the C-type CMS. To analyze the restorative genetic behavior of C-type CMS lines. The results showed that five inbred lines (5003,330, 330,035-6 and 6-11) were C-CMS lines, three inbred lines (32-2, Zhen367 and i-1) Maintaining sterility of C-CMS lines; sterility of Y-CMS lines were maintained in 6 inbred lines such as TR3702, 77-1, 10-9, 5003, 449, 1 was the restorer line of YⅡ CMS line, and the sterility of YⅡ type CMS line was maintained in 5 inbred lines such as TR3702, Qing2,0,05-6,330 and 5003. 32-2 pairs of C type, bearing 10-9 pairs of Y type, 5003 pairs of Y type and Y Ⅱ type of backcrossed F1 all sterile, continuous backcross method can be used to transform into a new sterile line. C-type CMS line F2 showed fertile and sterile 3: fertility segregation, backcross generation appeared 1: 1 fertility separation, indicating that the recovery by a dominant gene control.