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目的建立电感耦合等离子体质谱法(ICP-MS)快速测定全血中16种金属元素。方法全血样品经0.1%Triton X-100和1%硝酸的混合溶液直接稀释后,采用89Y、209Bi作为在线内标,经ICP-MS,采用碰撞反应池技术同时快速分析全血中的铅(Pb)、镉(Cd)、铍(Be)、钡(Ba)、锰(Mn)、铜(Cu)、锌(Zn)、钼(Mo)、钴(Co)、镍(Ni)、硒(Se)、砷(As)、锑(Sb)、钒(V)、银(Ag)、铬(Cr)含量。同时用全血痕量元素标准物质作质控样。结果该16种元素在对应的浓度范围内线性良好(相关系数均≥0.999 0),方法检出限为0.002μg/L~0.70μg/L,各元素的高、中、低3种浓度加标回收率为89.2%~113.5%,日间精密度为3.23%~10.96%,日内精密度为0.46%~9.29%。结论此方法简便、快速、准确,结果令人满意,适用于血液样品多种元素的分析测定,能满足生物样品的检测要求。
Objective To establish a rapid determination of 16 kinds of metal elements in whole blood by inductively coupled plasma mass spectrometry (ICP-MS). Methods Whole blood samples were directly diluted with a mixture of 0.1% Triton X-100 and 1% nitric acid, and 89Y and 209Bi were used as internal standard. The whole blood samples were analyzed by ICP-MS using collision cell technology Cadmium, beryllium, barium, manganese, copper, zinc, molybdenum, cobalt, nickel, selenium Se, As, Sb, V, Ag and Cr. At the same time with the whole blood trace element standard material for quality control samples. Results The 16 kinds of elements showed good linearity in the corresponding concentration range (all correlation coefficients ≥0.999 0). The detection limits were 0.002μg / L ~ 0.70μg / L, and the concentrations of three elements of high, medium and low were spiked The recoveries ranged from 89.2% to 113.5%, with intraday precision ranging from 3.23% to 10.96% and intraday precision ranging from 0.46% to 9.29%. Conclusion This method is simple, rapid, accurate and satisfactory. It is suitable for the analysis and determination of various elements in blood samples and can meet the testing requirements of biological samples.