论文部分内容阅读
目的：研究ＥＢＶ－ＬＭＰ及二甲基亚砜（ＤＭＳＯ）对人鼻咽癌（ＮＰＣ）细胞体外生长分化的影响。方法：以人高分化鼻咽癌细胞株（ＣＮＥ１）为对象，采用电穿孔基因转染技术，将重组ＥＢＶ－ＬＭＰ表达质粒转染ＣＮＥ１细胞，以载体质粒转染及ＣＮＥ１细胞为对照，用细胞体外增殖实验、流式细胞仪和免疫组化等方法，观察细胞生长分化的变化情况。结果：ＥＢＶ－ＬＭＰ在体外可明显促进ＣＮＥ１细胞的增殖，实验组与空白组及阴性对照组比较Ｐ＜０．０１，实验组Ｓ期细胞明显增多，细胞角蛋白表达显著下降（Ｐ＜０．０５），有向低分化鳞癌发展倾向；ＤＭＳＯ诱导后ＣＮＥ１细胞增殖力明显下降（Ｐ＜０．０１），细胞角蛋白表达显著增高（Ｐ＜０．０１）；ＬＭＰ可明显抑制ＤＭＳＯ对ＣＮＥ１细胞的诱导分化作用，转染细胞诱导后增殖力和角蛋白表达无显著改变（Ｐ＞０．０５）。结论：ＤＭＳＯ可在一定程度上诱导ＣＮＥ１细胞分化；ＥＢＶ－ＬＭＰ对ＣＮＥ１细胞生长有明显的促进作用，可抑制细胞分化及降低细胞对终末分化信号的反应，有助于深入研究ＮＰＣ的发生机理及防治
Objective: To investigate the effects of EBV-LMP and dimethyl sulfoxide (DMSO) on the growth and differentiation of human nasopharyngeal carcinoma (NPC) cells in vitro. METHODS: Human EBV-LMP expression vector was transfected into CNE1 cells using human hepatocellular carcinoma cell line (CNE1) and transfected with CNE1 by electroporation. The transfected cells were transfected with CNE1 cells and transfected with CNE1 cells. In vitro proliferation experiments, flow cytometry and immunohistochemistry and other methods to observe the changes in cell growth and differentiation. Results: EBV-LMP could significantly promote the proliferation of CNE1 cells in vitro. Compared with the blank control group and the blank control group, EBV-LMP significantly increased the number of S phase cells and the cytokeratin expression in the experimental group (P <0.01). (P <0.01). LMP could significantly inhibit the proliferation of CNE1 cells induced by DMSO (P <0.01) Cells induced differentiation, proliferation and keratin expression did not change significantly after transfected cells induced (P> 0.05). Conclusion: DMSO can induce the differentiation of CNE1 cells to a certain extent. EBV-LMP can significantly promote the growth of CNE1 cells, inhibit the differentiation of cells and decrease the response of cells to the signal of terminal differentiation, which is helpful to further study the mechanism of NPC And prevention