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目的:探讨阿司匹林对骨髓基质细胞成骨性分化的影响。方法:培养SD大鼠骨髓基质细胞(BMSCs),传代3次后进行成骨诱导分化,诱导培养基中加入不同浓度阿司匹林(0.5、1、2、5、10mmol/L),同时设立对照组。采用cck-8法分析细胞增殖情况。比较阿司匹林组与对照组在细胞碱性磷酸酶(ALP)活性、骨钙素(OC)分泌量、钙结节染色等方面的成骨性差异。结果:阿司匹林无促进细胞增殖活性,而高浓度阿司匹林能够强烈抑制细胞增殖。0.5、1、2mmol/L浓度阿司匹林可促进BMSCs的成骨性分化,中低浓度组碱性磷酸酶含量、骨钙素分泌量在不同阶段显著高于对照组。14天茜素红染色可见中低浓度组钙结节数量高于对照组。结论:中低浓度阿司匹林作用于骨髓基质细胞可促进其成骨细胞特性表达,这表明阿司匹林有促进骨代谢合成的作用。
Objective: To investigate the effect of aspirin on the osteogenic differentiation of bone marrow stromal cells. Methods: BMSCs were cultured in vitro and differentiated into osteoblasts after three passages. Induction of aspirin (0.5, 1, 2, 5 and 10 mmol / L) was induced in the culture medium. At the same time, the control group was established. Cck-8 method was used to analyze cell proliferation. The difference of osteoblasticity in ALP activity, osteocalcin (OC) secretion and calcium nodule staining between aspirin group and control group was compared. Results: Aspirin did not promote cell proliferation activity, while high concentrations of aspirin could strongly inhibit cell proliferation. 0.5, 1, 2mmol / L concentration of aspirin can promote the osteogenic differentiation of BMSCs, low and medium concentrations of alkaline phosphatase, osteocalcin secretion in different stages was significantly higher than the control group. 14 days Alizarin red staining shows that the number of calcium in the low-concentration group was higher than the control group. Conclusion: Aspirin can promote the expression of osteoblasts in low concentration aspirin, which indicates that aspirin can promote the synthesis of bone metabolism.