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采用Solixa高通量测序技术比较了1 000℃和1 800℃碳化麻秆制备电极OCP-1000和OCP-1800在生物电化学系统运行过程中形成的生物膜微生物群落结构差异.测序分别获得OCP-1000和OCP-1800附着生物膜16S rRNA基因V3区优化序列4 231和5 263条,经过97%相似度归并后获得OTUs数量分别为1 187和1 338个.α多样性指数(Chao、Shannon指数)分析表明碳化温度越低,电极生物膜微生物多样性越丰富.电极OCP-1000和OCP-1800生物膜中优势菌群均为Proteobacteria、Firmicutes和Bacteroidetes,这3个门的细菌在OCP-1000生物膜含量分别为66%、10%和9%,但在电极OCP-1800生物膜中含量分别为71%、7%和9%.不同碳化温度制备的麻秆电极在生物电化学系统运行过程形成的生物膜不仅存在共有细菌种类,也含有独特菌种,说明电极制备过程碳化温度影响电极生物膜微生物群落结构.
The microbial community structures of biofilms formed during the operation of bioelectrochemical system were compared by using Solixa high-throughput sequencing technology at 1 000 ℃ and 1 800 ℃, respectively.OCP-1000 and OCP- 1000 and OCP-1800 biosynthetic membrane 16S rRNA gene V3 region optimization sequence 4 231 and 5 263, after 97% similarity merger OTUs obtained were 1 187 and 1 338. α diversity index (Chao, Shannon index ) Analysis showed that the lower the carbonization temperature, the richer the microbial diversity of the electrode biofilm.The dominant bacteria in the OCP-1000 and OCP-1800 biofilms are Proteobacteria, Firmicutes and Bacteroidetes, The membrane contents were 66%, 10% and 9%, respectively, but were 71%, 7% and 9% respectively in the OCP-1800 biofilm.According to different carbonization temperatures, Of the biofilm not only exist in common bacterial species, but also contain unique species, indicating that the carbonization temperature of the electrode preparation process affects the biomembrane microbial community structure.