论文部分内容阅读
为了探索绵羊多胎性的分子机理以及为绵羊繁殖力的标记辅助选择和育种提供理论依据,采用PCR-RFLP及直接测序方法,对哈萨克羊群体BMP15基因Fec XI和Fec XH及第1内含子进行多态性检测。结果表明:BMP15基因Fec XI和Fec XH酶切位点均未出现多态性,在第1内含子存在2种基因型,AA型和AB型;优势基因型为AA型,优势等位基因为A基因,多态信息含量小于0.25,属于低度多态;χ~2适合性检验表明:处于Hardy-Weinberg平衡状态;测序结果表明:AB型个体在该基因第1内含子的第667位点发生G→T的突变,出现G/T的杂合。显著性检验分析表明:该突变位点在哈萨克羊群体中的产羔生产性能无显著性差异(P>0.05)。
In order to explore the molecular mechanism of multiple births in sheep and to provide a theoretical basis for marker-assisted selection and breeding of sheep fertility, PCR-RFLP and direct sequencing were performed on Fec XI, Fec XH and intron 1 of Kazak sheep population Polymorphism detection. The results showed that there was no polymorphism in the BMP15 gene Fec XI and Fec XH restriction sites, there were two genotypes in the first intron, AA and AB genotypes; the dominant genotype was AA, the dominant allele Because A gene, polymorphism information content is less than 0.25, belong to the low polymorphism; χ ~ 2 fitness test showed that: in the Hardy-Weinberg equilibrium state; sequencing results show: AB type in the first intron of the gene in the first 667 G → T site mutation occurs, there G / T hybrid. Significant test analysis showed that there was no significant difference in lambing performance among the Kazakh mutants (P> 0.05).