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目的探讨兔颈动脉外膜损伤后血管粥样硬化(AS)程度与动脉内去甲肾上腺素含量及交感神经密度的关系,并分析通心络对AS的作用效果及可能机制。方法采用Ⅰ型胶原酶局部损伤兔颈动脉外膜结合高脂喂养的方法 ,制备颈动脉外膜损伤致动脉粥样硬化模型,将模型动物随机分为2组:外膜损伤组、通心络治疗组;组内对左侧颈动脉行外膜损伤,右侧作为自身对照。分别于术后1、3、7d、2、4、8周取血及双侧颈动脉,采用酶联免疫吸附法(ELISA)检测兔血清及动脉壁中去甲肾上腺素含量,免疫组化法检测血管外膜交感神经密度。结果外膜损伤组左侧颈动脉内膜增生面积明显大于右侧,内膜增生面积与神经染色强度及局部动脉壁去甲肾上腺素含量呈正相关(r=0.94,P<0.05;r=0.90,P<0.05);与外膜损伤组相比,通心络能够明显减轻外膜损伤后血管内粥样斑块面积[IMR(48±2)%vs(70±3)%,P<0.01],降低动脉壁组织中去甲肾上腺素含量[(83.76±3.3)ng/gvs(74.25±2.1)ng/g,P<0.05]及血管的交感神经密度(P<0.05)。结论外膜损伤可加重高脂饮食诱导的AS病变程度,部分可能与其促进局部交感神经功能失调有关,通心络能够拮抗外膜损伤诱导的AS病变。
Objective To investigate the relationship between the degree of vascular atherosclerosis (AS) and norepinephrine content and sympathetic nerve density after carotid artery adventitia injury in rabbits and to analyze the effect of Tongxinluo on AS and its possible mechanism. Methods The model of atherosclerosis induced by adventitia of carotid artery was prepared by carotid adventitia and hyperlipidemic feeding in rabbits. The model animals were randomly divided into 2 groups: epicardial injury group, Tongxinluo In the treatment group, the left carotid artery was treated with adventitial injury and the right as the control. Blood and bilateral carotid arteries were harvested at 1, 3, 7d, 2, 4, and 8 weeks after surgery. The levels of norepinephrine in serum and arterial wall were detected by enzyme-linked immunosorbent assay (ELISA) Detection of vascular adventitial sympathetic density. Results The area of carotid intima hyperplasia on left carotid artery was significantly larger in right adventitial injury group than in right carotid artery. The area of intimal hyperplasia was positively correlated with nerve staining intensity and norepinephrine level in local arterial wall (r = 0.94, P <0.05; r = 0.90, Compared with the injury group, Tongxinluo can obviously reduce the area of atherosclerotic plaque after the injury of the outer membrane [IMR (48 ± 2)% vs (70 ± 3)%, P <0.01] (83.76 ± 3.3) ng / gvs (74.25 ± 2.1) ng / g, P <0.05], and sympathetic densities of blood vessels (P <0.05). Conclusion Outer membrane injury can aggravate the severity of AS induced by high-fat diet, which may partly be related to the promotion of local sympathetic dysfunction. Tongxinluo can antagonize the injury of AS induced by outer membrane injury.