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目的现场评价虚拟浓缩器采集微生物气溶胶的性能。方法在公共场所现场条件下,使用Biosampler和虚拟浓缩器与Biosampler采样器联用两种采样方法,分别采集军团菌气溶胶以及甲型和乙型流感病毒气溶胶,以巢式PCR扩增嗜肺军团菌Mip基因,一步法逆转录巢式PCR扩增甲型流感病毒基质蛋白基因和乙型流感病毒核蛋白基因,琼脂糖凝胶电泳检测扩增产物。结果共采集军团菌气溶胶样本98份,流感病毒气溶胶样本32份。虚拟浓缩器与Biosampler联用和Biosampler单独使用采集嗜肺军团菌的阳性率分别为12.2%(6/49)和18.4%(9/49),甲型流感病毒的阳性率分别是33.3%(4/12)和25.0%(5/20),乙型流感病毒阳性率分别为41.7%(5/12)和40.0%(8/20)。两种采样方法采集的嗜肺军团菌和流感病毒阳性率差异均无统计学意义。结论虚拟浓缩器可起到浓缩微生物气溶胶的作用,与Biosampler微生物采样器联用可为快速高效地采集低浓度微生物气溶胶提供技术手段。
Objective To evaluate the performance of virtual concentrator in collecting microbial aerosol. Methods In the field of public places, Biosampler, virtual concentrator and Biosampler sampler were used in combination with two sampling methods to collect aerosols of Legionella as well as influenza viruses A and B respectively. Legionella Mip gene, one-step reverse transcription nested PCR amplification of influenza A virus matrix protein gene and influenza B virus nucleoprotein gene, amplification products were detected by agarose gel electrophoresis. Results A total of 98 aerosol samples of Legionella were collected and 32 samples of influenza virus aerosol were collected. The positive rates of virtual concentrator with Biosampler and Biosampler alone were 12.2% (6/49) and 18.4% (9/49), respectively, and the positive rates of influenza A virus were 33.3% (4 / 12) and 25.0% (5/20) respectively. The positive rates of influenza B virus were 41.7% (5/12) and 40.0% (8/20), respectively. There was no significant difference in the positive rates of Legionella pneumophila and influenza virus between the two sampling methods. Conclusion The virtual concentrator can act as a concentrated microbial aerosol. Combined with the Biosampler microbial sampler, it can provide technical means for collecting low-concentration microbial aerosols quickly and efficiently.