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采用电化学氧化法将2-氨基乙硫醇共价键合在玻碳电极表面,并采用X-射线光电子能谱和电化学方法对其进行了表征.2-氨基乙硫醇修饰的玻碳电极对尿酸显示出较好的催化氧化作用,表现在尿酸氧化过电位的负移(230 mV)和峰电流的增加.并且,尿酸的氧化峰电流在8.0×10-6~2.0×10-4mol/L内随尿酸浓度的增加而线性增大,相关系数为0.9976,检测限为4.8×10-7mol/L.该法被成功地用于人尿中尿酸含量的测定.另外,该修饰电极可将常规电极上尿酸和抗坏血酸重叠的氧化波进行有效的分离,由此,该法有望用于两者的同时测定.
2-Aminoethanethiol was covalently bonded to the surface of glassy carbon electrode by electrochemical oxidation method and characterized by X-ray photoelectron spectroscopy and electrochemical method .2-Aminoethanethiol modified glassy carbon The electrode shows good catalytic oxidation for uric acid, showing a negative shift (230 mV) and peak current of uric acid oxidation overpotential, and the oxidation peak current of uric acid is in the range of 8.0 × 10 -6 to 2.0 × 10 -4 mol / L linearly increased with the increase of uric acid concentration, the correlation coefficient was 0.9976, the detection limit was 4.8 × 10-7mol / L. This method was successfully used for the determination of uric acid in human urine.In addition, the modified electrode The conventional electrode of uric acid and ascorbic acid overlap of the oxidation of the effective separation, which is expected to be used for both the simultaneous determination of both.