论文部分内容阅读
本文观察PMB及其模拟肽处理前后FITC-LPS与J774A1的结合能力及CD14表达,并检测培养上清中细胞因子TNF-α、IL-6的含量变化。结果发现①FITC-LPS分别与PMB、peptide 1孵育后,细胞膜平均通道荧光显著减少,LPS与J774A1的结合能力显著降低;②100 ng/ml LPS刺激J774A1 3 h后CD14阳性率明显增加;LPS分别与PMB和peptide 1预孵育后可显著降低LPS刺激J774A1细胞膜CD14表达;③LPS刺激J774A1分泌TNF-α和IL-6显著增加,LPS分别与PMB和petide 1预孵育后能显著减少细胞因子TNF-α和IL-6分泌。结果证实多粘菌素B及其模拟肽(peptide 1)可能通过下调J774A1 CD14表达,降低细胞因子水平来减少LPS诱导的炎症反应。
In this paper, the binding ability of FITC-LPS to J774A1 and the expression of CD14 before and after treatment with PMB and its mimic peptide were observed. The contents of cytokines TNF-α and IL-6 in culture supernatants were detected. The results showed that (1) FITC-LPS incubated with PMB and peptide 1 decreased the mean fluorescence of cell membrane and the binding ability of LPS to J774A1 significantly; ② The positive rate of CD14 increased obviously after 3 hours stimulation with 100 ng / ml LPS; Preincubation with peptide 1 significantly reduced CD14 expression in J774A1 cells stimulated by LPS; ③ TNF-α and IL-6 secreted by LPS-stimulated J774A1 significantly increased; LPS pretreatment with PMB and petide 1 significantly decreased cytokine TNF-α and IL-6 -6 secretion. The results confirmed that polymyxin B and its peptide (peptide 1) may reduce the LPS-induced inflammatory response by down-regulating J774A1 CD14 expression and decreasing cytokine levels.