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用10种不同浓度的阿特拉津溶液对津稻107进行叶片涂抹处理,从而确定可以用产生药害的0.076%～0.190%浓度(W V)的阿特拉津溶液检测转化植株对除草剂的抗性。用以上浓度范围的阿特拉津溶液涂抹处理津稻107转化植株,并对在此浓度下检测出的除草剂抗性和敏感的转化植株进行了PCR分析,结果表明,除草剂抗性鉴定结果与分子检测结果一致。因此可以利用浓度为0.076%～0.190%的阿特拉津溶液对早期转化株进行叶片涂抹法鉴定。
Ten leaves of Atosium 107 were leaf-coated with 10 different concentrations of atrazine in order to determine the effect of the atrazine on the herbicidal activity of the transformed plants at a concentration of 0.076% ~ 0.190% (WV) Resistant. PCR was carried out with the atrazine solution of the above concentration range on the plants transformed with Tsugai107 and the PCR analysis of the herbicide resistant and susceptible transformed plants detected at this concentration showed that the result of the herbicide resistance identification Consistent with the molecular test results. Therefore, we can use the concentration of 0.076% ~ 0.190% of atrazine solution of early transformants were identified by leaf smear.