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运用噬菌体表面表达（Ｐｈａｇｅｄｉｓｐｌａｙ）技术，获得人源中和性抗汉滩病毒汉滩型Ｇ１基因工程单克隆抗体（单抗）Ｆａｂ段基因及其表达，并同时获得抗汉滩病毒核蛋白（ＮＰ）的Ｆａｂ抗体。从肾综合征出血热疫区恢复期病人抗凝血中分离到的外周淋巴细胞中，提取了总细胞ＲＮＡ。通过ＲＴ－ＰＣＲ方法，用一组人ＩｇＧＦａｂ基因特异性引物，从合成的ｃＤＮＡ中经ＰＣＲ扩增了一组轻链和重链Ｆａｂ段基因，将轻链和重链先后插入噬菌体载体ｐＣｏｍｂ３，成功地建立了抗汉滩病毒抗体基因库，并用纯化的汉滩病毒颗粒及抗汉滩病毒糖蛋白鼠单抗捕捉糖蛋白抗体原的方法，对此抗体库进行了富集筛选，在短期内成功地获得了抗汉滩病毒核蛋白和糖蛋白Ｇ１的人源单抗Ｆａｂ段基因，并在大肠杆菌中获得有效表达。核苷酸序列分析证实，所获得的基因为人源ＩｇＧＦａｂ基因。用特异性放射免疫沉淀（ＩＰ）、ＩＦＡＴ和ＥＬＩＳＡ以及空斑减少中和试验鉴定表明，表达的人Ｆａｂ抗体能识别汉滩病毒结构蛋白，其中抗Ｇ１人Ｆａｂ抗体具有体外中和活性。人源抗汉滩病毒基因工程抗体的获得，为今后可能的临床应用提供了良好前景。
The Fab gene of human-derived anti-Hantaan hanotype G1 genetically engineered monoclonal antibody (McAb) and its expression were obtained by using phaged surface expression (Phagedisplay) technique. Meanwhile, the anti-Hantaan virus nucleoprotein ) Of Fab antibody. Total cellular RNA was extracted from peripheral blood lymphocytes isolated from patients with anticoagulant hemorrhagic fever with renal syndrome. A group of human IgGFab gene-specific primers were used to amplify a group of light chain and heavy chain Fab fragment genes from the synthesized cDNA by RT-PCR. The light and heavy chains were inserted into the phage vector pComb3 successfully Established anti-Hantaan virus antibody gene library, and purified antibody and anti-Hantaan virus glycoprotein mouse monoclonal antibody capture glycoprotein antibody method, the antibody library enrichment screening, in the short term success The Fab fragment of human monoclonal antibody against Hantaan virus nucleoprotein and glycoprotein Gl was obtained and expressed efficiently in E. coli. Nucleotide sequence analysis confirmed that the obtained gene was human IgGFab gene. Identification by specific radioimmunoprecipitation (IP), IFAT and ELISA and plaque reduction neutralization assays showed that the expressed human Fab antibody recognized the Hantaan virus structural protein, wherein the anti-G1 human Fab antibody had in vitro neutralizing activity. The availability of human anti-Hantaan virus genetically engineered antibodies provides good prospects for possible future clinical applications.