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为探索人脑髓鞘碱性蛋白（ＭＢＰ）基因表达的时空秩序与髓鞘发生和形成之间关系，采用３２Ｐ－和生物素标记鼠ＭＢＰｃＤＮＡ和地高辛标记人ＭＢＰｃＲＮＡ探针，对发育过程中人脑进行Ｎｏｒｔｈｅｒｎ斑点杂交和原位杂交研究。Ｎｏｒｔｈｅｒｎ斑点分析结果显示：１６周胎龄脑ＭＢＰｍＲＮＡ表达已明显，Ａ５９０值近２．０／μｇＲＮＡ，随发育逐渐增加，至成年其表达增至９倍（Ａ５９０值为１８／μｇＲＮＡ）。８～２５周胎龄脊髓和延脑切片以及２０、２２和２５周胎龄桥脑、中脑、丘脑、小脑和大脑切片原位杂交结果表明：在８～９周胎龄人脑脊髓前角和近延脑第四脑室底区域中，其散在细胞的核周胞浆内有ＭＢＰｍＲＮＡ表达信号，该阳性细胞比少突细胞大，而且胞浆丰富，它们究竟是神经元或胶质前体仍待鉴定。在２０周胎龄期，ＭＢＰｍＲＮＡ表达在脊髓和延脑区骤增，并沿少突细胞突起延伸呈网状；此期桥脑该基因表达已明显，在其他脑区该表达信号也有散在分布
In order to explore the relationship between the spatial and temporal order of myelin basic protein (MBP) gene expression and myelination and formation, 32P- and biotinylated mouse MBP cDNA and digoxigenin-labeled human MBPcRNA probe were used to detect Brain Northern blotting and in situ hybridization studies. The result of Northern blot analysis showed that the expression of MBP mRNA in the brain of 16 weeks gestation was obvious. The A590 value was nearly 2.0 / μg RNA, which gradually increased with the development. The expression of A590 increased to 9 times (A590 18 / μg RNA). The results of in situ hybridization between 8 ~ 25 weeks gestational age spinal cord and extensor brain slices, and 20, 22 and 25 weeks gestational age in pontine, midbrain, thalamus, cerebellum and brain slices showed that at 8-9 weeks gestational age, And near the middle of the fourth ventricle near the brain area, the scattered nuclei in the cytoplasm of cells in the MBPmRNA expression signal, the positive cells than the oligodendrocytes, and abundant cytoplasm, whether they are neurons or glial precursors To be identified. MBP mRNA expression increased sharply in the spinal cord and medulla oblongata at 20 weeks gestational age and reticularly along the process of protrusion of the oligodendrocytes. The expression of MBP mRNA was apparent in this period, and the expression signal was scattered in other brain regions