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三氯醋酸产生于工业废弃物及水的氯化过程 ,是重要的环境污染物 .对于它的检测 ,常用的方法比较繁琐 .应用血红蛋白 -蒙脱土膜修饰热解石墨电极 ,将血红蛋白包埋于修饰在热解石墨电极上的蒙脱土内 ,进行循环伏安实验 ,可以得到血红蛋白的直接电化学响应 ,在pH 4.0 0 .1mol/L柠檬酸缓冲液中 ,于- 0 .2 92V处出现一对准可逆的氧化还原峰 (相对于饱和甘汞电极 ,VS.SCE) .经紫外 -可见光谱和傅利叶红外光谱测定 ,包埋在蒙脱土膜内的血红蛋白仍保持与其天然状态相似的二级结构 ,而且将三氯醋酸加入上述体系中 ,发现该修饰电极对三氯醋酸具有催化功能 .表观米氏常数的测定结果显示 ,该参数可作为这种电极与底物三氯醋酸作用的酶性质的表征 .同时血红蛋白 -蒙脱土膜修饰热解石墨电极性质稳定 ,对于三氯醋酸 ,显示出了酶样的催化作用特性 ,特异性高 .因此水中虽然存在一些浓度高于三氯醋酸三倍的相关干扰物质的同时 ,该电极仍显示出了良好的选择性 ,并具有方便、快捷的特点 .还从电化学的角度出发 ,探讨了血红蛋白 -蒙脱土膜的修饰电极在环境污染物三氯醋酸检测中应用的可能性
Trichloroacetic acid, which is produced in industrial waste and chlorination of water, is an important environmental pollutant.As for its detection, the commonly used method is cumbersome.Hemoglobin is embedded by hemoglobin-montmorillonite modified pyrolytic graphite electrode Cyclic voltammetry experiments were performed on montmorillonite modified on pyrolytic graphite electrodes to obtain the direct electrochemical response of hemoglobin at -0.22 V at pH 4.0 0 .1 mol / L citric acid buffer A quasi-reversible redox peak appeared (vs. VS.SCE vs. saturated calomel electrode). The hemoglobin embedded in the montmorillonite membrane remained similar to its natural state as measured by UV-Vis and Fourier transform infrared spectroscopy Secondary structure, and the trichloroacetic acid was added to the above system and found that the modified electrode has catalytic function of trichloroacetic acid.Measurement of the apparent Michaelis constant results show that this parameter can be used as the electrode and the substrate trichloroacetic acid role The characterization of the enzymatic properties of the hemoglobin-montmorillonite modified pyrolytic graphite electrode was stable, and for the trichloroacetic acid, it showed the characteristic of enzyme-like catalysis and high specificity Despite the presence of some interfering substances at concentrations three times higher than that of trichloroacetic acid, the electrode showed good selectivity and was convenient and fast.Furthermore, the electrochemical properties of hemoglobin- The possibility of using modified electrode of soil-separating membrane in the detection of environmental pollutant trichloroacetic acid