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为探讨母体铅暴露对仔鼠肝脏MMP-2和MMP-9 mRNA表达的影响,采用自由饮水模式建立铅暴露动物模型,将40只雌性小鼠自妊娠第1天开始经饮水染铅(1.0,5.0,10.0g/L,对照组饮蒸馏水)至仔鼠出生后21d,随机分为低、中、高剂量染毒组和对照组,仔鼠21日龄,分别测其血液和肝脏中铅的含量,然后取其肝脏组织,通过实时荧光定量PCR(Real-time PCR)技术检测各组仔鼠肝脏中MMP-2和MMP-9mRNA的表达情况。结果显示,21d后,铅暴露组仔鼠血液和肝脏中铅水平均明显高于对照组(P<0.05);与对照组相比,低、中和高剂量铅暴露组仔鼠肝脏内MMP-2mRNA的表达量明显增高,且差异具有统计学意义(P<0.05)。中、高剂量铅暴露组仔鼠肝脏组织中MMP-9mRNA的表达明显高于对照组(P<0.05),但低剂量铅暴露组仔鼠肝脏组织中MMP-9mRNA的表达与对照组相比差异不显著(P>0.05)。结果表明,母体铅暴露可能通过改变仔鼠肝脏中MMP-2 mRNA的表达进而造成肝脏损伤。母体铅暴露使铅在仔鼠体内蓄积,铅暴露可能通过增强仔鼠肝脏中MMP-9mRNA的表达进而造成肝脏损伤,从而引起组织发生病变。
In order to investigate the effect of maternal lead exposure on mRNA expression of MMP-2 and MMP-9 in offspring liver, animal models of lead exposure were established by using free drinking water model. 40 female mice were infected with lead (1.0, 5.0, 10.0g / L, distilled water in the control group) to the pups 21d after birth, were randomly divided into low, medium and high dose exposure groups and control groups, pups 21 days old, were measured in the blood and liver lead The liver tissue was harvested for detection of the expression of MMP-2 and MMP-9 mRNA in the livers of the pups in each group by Real-time PCR. The results showed that after 21 days, lead levels in blood and liver were significantly higher in the lead exposure group than in the control group (P <0.05). Compared with the control group, the levels of MMP- 2 mRNA expression was significantly increased, and the difference was statistically significant (P <0.05). The expression of MMP-9mRNA in liver tissue of medium and high-dose lead exposed group was significantly higher than that of the control group (P <0.05), but the expression of MMP-9mRNA in liver tissue of low dose lead exposed group was significantly lower than that of the control group Not significant (P> 0.05). The results showed that maternal lead exposure may cause liver damage by altering MMP-2 mRNA expression in the offspring liver. Exposure to lead by maternal lead accumulates lead in the offspring, and lead exposure may cause liver damage by increasing MMP-9 mRNA expression in the offspring of the offspring, causing lesions in the tissue.