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高粱是一种抗旱性较强的禾谷类作物。本研究在高粱中克隆到一个全长为693 bp的编码ATP合成酶E亚基的基因(SbATPase-E)。在高粱幼苗期,SbATPase-E基因受Na Cl和脱落酸(ABA)处理诱导上调表达。该基因在拟南芥中过量表达可提高转基因植株的耐旱性和耐盐性,在逆境胁迫条件下转基因拟南芥植株较野生型植株根系发达,可能是转基因植株耐旱性和耐盐性提高的主要原因。在干旱胁迫条件下,转基因植株中DREB2A、P5CS1、RD29A、RAB18和ABI1基因的表达量相对于野生型植株中的表达量提高更为显著;在高盐处理条件下,转基因植株中SOS1和SOS2基因的表达量也较野生型植株中的表达量明显提高。这些抗逆相关基因的上调表达可能是转基因植株抗逆性提高的主要分子机制。
Sorghum is a drought-tolerant cereal crop. In this study, a full-length 693 bp ATPase synthase E subunit gene (SbATPase-E) was cloned from sorghum. In sorghum seedling stage, SbATPase-E gene was up-regulated by NaCl and abscisic acid (ABA) treatment. The overexpression of this gene in Arabidopsis could increase the drought tolerance and salt tolerance of transgenic plants. Transgenic Arabidopsis plants developed roots under wild stress compared with wild type plants, which may be due to the drought tolerance and salt tolerance of transgenic plants The main reason for improvement. Under drought stress, the expression of DREB2A, P5CS1, RD29A, RAB18 and ABI1 in transgenic plants increased more significantly than that in wild type plants. Under high salt stress, the expression of SOS1 and SOS2 genes in transgenic plants The expression level of wild-type plants also significantly increased. Up-regulated expression of these anti-stress-related genes may be the main molecular mechanism for the improvement of stress tolerance in transgenic plants.