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对造成香菇原生质体单核化后两种交配型单核体比例偏离1∶1分离的遗传背景进行探讨.结果表明,B因子或是与B因子连锁的因子对这种偏离有明显的影响,而A因子对此影响不明显.B因子特异性不同,与之相对应的交配型单核体出现的比例亦不同,特异性越高,其对应交配型单核体在经原生质体单核化后将以较大的比例出现.参试的2个香菇菌株中4个B因子特异性顺序为B1>B3>B4>B2.双-单杂交所形成的由受体细胞质和受体、供体细胞核组成的同质异核体经原生质体单核化裂解后均与受体交配型相同的核居多,表明受体细胞质环境对双-单杂交所得异核体裂解后的核的存活和数量起着重要作用.作为供体的2个核中只有亲和力强的那个核优先迁移到受体中,形成新的异核体,即存在先导核.先导核与B因子的亲和特异性有关,特异性越高,先导核迁移速度越快.
The genetic background of the segregation ratio of the two mating type mononuclear bodies after the single nucleation of the protoplast of Mushroom was explored.The results showed that the factor B or the factor linked with the B factor had obvious influence on this deviation, While the A factor has no obvious effect on this factor.B factor specificity is different, corresponding to the mating type of mononuclear also appear in different proportions, the higher the specificity, the corresponding mating type mononuclear in protoplast mononuclear Will appear in larger scale.The specific sequence of the four B factors in the two lentinan strains tested is B1> B3> B4> B2. The double-mono hybrid formed by the receptor cytoplasm and the receptor, donor The nuclei composed of homogeneous heterokaryons protoplast lynuclein cleavage were the same type of receptor mating type nuclear majority, indicating that the recipient cytoplasmic environment of double - single hybrid heterokaryon lysis after the survival and the number of nuclear Plays an important role in the two nuclear as the donor only the affinity of the nuclear preferential migration to the receptor to form a new heterokaryon, that is, there is a precursor nucleus.The precursor nucleus and the affinity of the B-specific specificity, specificity The higher the sex, the faster the lead nuclear migration.