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目的:探讨研究白芷苗期抗旱性的最佳模拟条件,对白芷苗期抗旱性进行综合评价,并筛选鉴定白芷苗期抗旱性指标。方法:采用5种不同浓度聚乙二醇(PEG-6000)分别胁迫白芷幼苗3,6,9,12 d,测定其株高、根长、根冠比、可溶性糖、脯氨酸和丙二醛等。用隶属函数法评价白芷药材不同原植物的抗旱性,用灰色关联度分析法筛选白芷苗期抗旱鉴定指标。结果:以20%的PEG胁迫9 d可作为研究白芷苗期抗旱性的最佳模拟条件。根据隶属函数均值进行抗旱性评价,抗旱性由强到弱依次为:川白芷、禹白芷、祁白芷和杭白芷。9个考察指标与隶属函数均值的关联序为:可溶性糖>根长>脯氨酸>根冠比>叶绿素总含量>叶绿素b>叶绿素a>株高>丙二醛。结论:PEG-6000模拟干旱条件下,渗透调节物质以及根的相关指标对白芷苗期抗旱性影响较大,川白芷苗期抗旱性最强。
OBJECTIVE: To explore the best simulation conditions for studying the drought resistance of Angelicae Dahuricae at seedling stage, to evaluate the drought resistance of Angelicae Dahuricae at seedling stage and to screen the drought resistance index of Angelica Dahuricae at seedling stage. Methods: Different concentrations of polyethylene glycol (PEG-6000) were used to intimidate Angelica sinensis seedlings for 3, 6, 9 and 12 days respectively. The plant height, root length, ratio of root and shoot, soluble sugar, proline and Aldehyde and so on. The membership function method was used to evaluate the drought resistance of different plants of Angelica dahurica. Gray relational analysis was used to screen the drought resistance index of Angelica dahurica seedlings. Results: Drought stress of Angelica indica at seedling stage was best simulated with 20% PEG for 9 d. Drought resistance was evaluated according to the mean value of membership functions. The order of drought resistance from strong to weak was Chuan Baizhi, Yu Baizhi, Qi Baizhi and Hangbaizhi. The correlation order of nine indicators with the membership function was: soluble sugar> root length> proline> root / shoot ratio> total chlorophyll content> chlorophyll b> chlorophyll a> plant height> malondialdehyde. Conclusion: PEG-6000 under simulated drought conditions, osmotica and root-related indicators of the greater impact on the drought resistance of Angelica at seedling stage, Chuanbazhizao strongest drought resistance at seedling stage.