论文部分内容阅读
目的 观察重组 IL-2、IL-6和 TNF-α(r IL-2 ,r IL-6,r TNF-α)作为血吸虫病抗独特型抗体疫苗佐剂的可行性 ,并探讨其作用机制。方法 日本血吸虫抗独特型抗体 NP3 0主动免疫BAL B/c小鼠 ,分别联用 r IL-2、r IL-6和 r TNF-α,观察其对小鼠的保护性免疫作用 ,以及对宿主体液免疫和细胞免疫的影响。结果 r IL-2和 r IL -6能明显提高 NP3 0疫苗对小鼠宿主的保护性作用 ,减虫率和减卵率均明显提高 ,减虫率从单用 NP3 0的 40 .6%分别提高到 5 3 .5 %和 5 5 .7% ,减卵率从 46.5 %分别提高到 68.7%和 69.8% :二者均使血清特异性 Ig G、Ig G1和 Ig G2 a抗体明显升高 ;另外 ,足垫试验结果显示 NP3 0加用 r IL -2可引发迟发型变态反应。 r TNF -α无增强 NP3 0的保护性免疫作用。结论 r IL -2和 r IL -6可作为血吸虫病抗独特型抗体疫苗的佐剂 ,其作用机制与同时增强 Th1和 Th2免疫应答有关
Objective To observe the feasibility of recombinant IL-2, IL-6 and TNF-α (rIL-2, rIL-6, r TNF-α) adjuvant for schistosoma japonicum anti-idiotypic antibody vaccine and to explore its mechanism. Methods BALB / c mice were immunized with the anti-idiotypic antibody NP30 of Schistosoma japonicum. The protective immunity of mice was evaluated by r IL-2, r IL-6 and r TNF-α respectively. Humoral and cellular immunity. Results r IL-2 and r IL-6 could significantly improve the protective effect of NP3 0 vaccine on the host mice, the worm reduction rate and the egg reduction rate were significantly increased, the reduction rate from 40.6% Increased to 53.5% and 55.7% respectively, and the oviposition rate increased from 46.5% to 68.7% and 69.8% respectively, both of which increased the serum specific Ig G, Ig G1 and Ig G2 a antibodies. In addition, foot pad test results show that NP3 0 plus r IL -2 can trigger delayed-type hypersensitivity. TNF-α does not enhance the protective immunity of NP3 0. Conclusion r IL -2 and r IL -6 can be used as an adjuvant for anti-idiotypic antibody vaccine against schistosomiasis, and its mechanism of action is related to the simultaneous enhancement of Th1 and Th2 immune responses