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目的 探讨磁场对肿瘤细胞凋亡及其相关基因 Bcl- 2及 Bax蛋白表达的影响 ,揭示磁场抗癌作用的分子机制。方法 460只 SD大鼠右下肢皮下移植 Walker- 2 56瘤株 ,常规饲养 2周后 ,随机选择 40 0只分为荷瘤对照组和荷瘤实验组。实验组每日接受不同强度不同时间的磁场处理 ,持续 2周 ,观察瘤鼠存活率 ,然后处死 ,测量瘤体体积及湿重 ,最后行 HE及免疫组化染色。结果 与对照组比较 ,实验组大鼠存活率 (84.2 8% )增高 ,肿瘤体积 (1 .78cm3± 0 .82 cm3)及肿瘤湿重 (2 .2 0 g± 1 .43g)明显减小 (P <0 .0 5)。病理组织学检查可见细胞凋亡的数目明显增多。Bcl- 2蛋白阳性表达率 (1 8% )低于对照组 ,Bax蛋白阳性表达率 (43% )高于对照组 (P <0 .0 5) ,Bcl- 2 /Bax的比值低于对照组。结论 恒定均匀磁场的作用结果使凋亡相关基因 Bcl- 2蛋白的表达下调 ,Bax蛋白的表达增高 ,Bcl- 2 /Bax的比值减小 ,是诱导肿瘤细胞凋亡的原因之一
Objective To investigate the effect of magnetic field on the apoptosis of tumor cells and the expression of related genes Bcl-2 and Bax, and to reveal the molecular mechanism of the anti-cancer effects of magnetic fields. Methods A total of 460 SD rats were transplanted with Walker-256 tumor subcutaneously in the right lower extremity. After 2 weeks of routine feeding, 400 rabbits were randomly divided into tumor-bearing control group and tumor-bearing experimental group. The experimental group received a magnetic field treatment of different intensity for different periods of time every day for 2 weeks. The survival rate of the tumor mice was observed, and then sacrificed. The tumor volume and wet weight were measured, and HE and immunohistochemical staining were performed. Results Compared with the control group, the survival rate of the experimental group (84.28%) increased, and the tumor volume (1.78cm3±0.82cm3) and the wet weight of the tumor (2.20±1.43g) were significantly reduced ( P <0. 0 5). Histopathological examination showed a significant increase in the number of apoptotic cells. The positive expression rate of Bcl-2 protein (18%) was lower than that of the control group. The positive expression rate of Bax protein was higher (43%) than that of the control group (P < 0.05). The ratio of Bcl-2/Bax was lower than that of the control group. . Conclusion The effect of a constant uniform magnetic field results in the down-regulation of apoptosis-related gene Bcl-2, increased expression of Bax protein, and reduced Bcl-2/Bax ratio, which is one of the causes of apoptosis of tumor cells.