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A novel quinoline derivative 6-(10-thiol-decaoxy ) quinoine (abbreviated as QC10SH )was synthesized and its seif-assembled monoayers (SAMs) were prepared on gold for the first time. Wettability measurement on QC10SH SAM reveals a hydrophilic surface. RA-IR researches suggests that the SAM has formed highly-organized and closely-packed structure. A method is developed for quanitatively evaluating the molecular orientation in monoayer. By this method the result that the twisting and tilting angles of QC10SH moecule in SAM are 50±4° and 24±3°respectively has been obtained.