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目的探讨碘对小鼠树突状细胞(dendritic cell,DC)前体表型及功能的影响。方法选雌性C57BL/6 J小鼠40只,按碘摄入量随机分为适碘组(NI)、低碘组(LI)、10倍碘过量组(10H I)、50倍碘过量组(50H I)4组,喂养8个月后检测小鼠DC表面分子表达和培养上清中白细胞介素-12(interleukin-12,IL-12)水平以及DC刺激T细胞的增殖能力。结果 LI组小鼠DC表面分子CD40+CD11 c+、CD80+CD11 c+、CD86+CD11 c+、MHC-II+CD11 c+在CD11 c+细胞中所占比例均低于NI组小鼠,差异均有统计学意义(P<0.05);50H I组小鼠DC表面分子CD40+CD11 c+、CD80+CD11 c+、CD86+CD11 c+、MHC-II+CD11 c+在CD11 c+细胞中所占比例均高于NI组小鼠,差异均有统计学意义(P<0.05);LI组小鼠DC培养上清中细胞因子IL-12浓度为(275.8±32.6)pg/mL,低于NI组小鼠的(548.7±51.3)pg/mL(P<0.01);50H I组小鼠DC培养上清中细胞因子IL-12浓度为(963.2±83.4)pg/mL,高于NI组小鼠(P<0.01);LI组的刺激指数(SI)为(5.23±1.96),低于NI组小鼠的(8.61±2.15)(P<0.05);50H I组小鼠的SI为(12.38±2.61),高于NI组小鼠(P<0.05)。结论摄入一定水平的碘可诱导小鼠DC前体的增殖和成熟,提高机体的免疫功能。
Objective To investigate the effects of iodine on the phenotype and function of dendritic cell precursors in mice. Methods Forty female C57BL / 6 J mice were randomly divided into five groups according to iodine intake: NI, LI, 10H I, 50D iodine excess 50H I). After 8 months of feeding, the expression of DCs on the surface of DCs in mice and the level of interleukin-12 (IL-12) in the culture supernatant and the ability of DCs to stimulate the proliferation of T cells were detected. Results The percentage of CD40 + CD11 c +, CD80 + CD11 c +, CD86 + CD11 c +, MHC-II + CD11 c + on CD11 c + cells in LI group was lower than that in NI group (P <0.05). The proportion of CD40 + CD11 c +, CD80 + CD11 c +, CD86 + CD11 c + and MHC-II + CD11 c + on CD11 c + cells of DCs in 50H I group was higher than that in NI group (P <0.05). The concentration of IL-12 in the culture supernatant of LI group was (275.8 ± 32.6) pg / mL, which was lower than that in NI group (548.7 ± 51.3 ) pg / mL (P <0.01). The concentration of cytokine IL-12 in the culture supernatant of DCs in 50H I group was (963.2 ± 83.4) pg / mL, (SI) was (5.23 ± 1.96), which was lower than that of NI group (8.61 ± 2.15) (P <0.05). The SI of group 50H I was (12.38 ± 2.61), which was higher than that of NI group Mouse (P <0.05). Conclusion Ingesting a certain level of iodine can induce the proliferation and maturation of DC precursors in mice and improve the body’s immune function.