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目的了解上世纪70~90年代我国农村单采浆献血者感染输血后丙型肝炎病毒后的现状及其HCV感染与疾病转归的相关性。方法调查河北省某地农村229名单采浆献血者HCV感染后的现状,包括血清ALT、病毒学标志检测,其中HCV RNA的测定采用荧光定量PCR方法,抗-HCV检测采用ELISA法,血清ALT检测采用赖氏法。结果 229名农村单采浆献血者感染输血后HCV的慢性化率为57.64%;ALT异常率HCV RNA阳性组为46.97(62/132)、阴性组4.129(4/57)(P<0.01);ALT异常率抗-HCV阴性组、抗-HCV低滴度组对比数据及高滴度组分别为0、33.80%及42.84%(P<0.01)。结论迄今为止我国农村单采浆献血者感染HCV的表现仍为慢性、隐匿性,肝酶学检查指标大多轻度到中度异常,随着HCV RNA载量增加抗-HCV阳性检出率和ALT的异常率增高,因此三者联合检测在正确诊断和预测他们的肝脏损伤及早期疗效观察中具有重要的意义。
Objective To understand the status of post-transfusion hepatitis C virus infection and the correlation between HCV infection and the outcome of the disease in rural area of China in the 1990s. Methods The status of HCV infection in 229 plasmapheresis donors from a rural area in Hebei Province was investigated. The serum ALT and virological markers were detected. HCV RNA was detected by real-time PCR, anti-HCV was detected by ELISA and serum ALT Lai’s method. Results The 229 HCV infection rate was 57.64%. The abnormal rate of ALT was 46.97 (62/132) in HCV RNA positive group and 4.129 (4/57) in negative group (P <0.01). The data of ALT abnormality anti-HCV negative group and anti-HCV low titer group were 0, 33.80% and 42.84% respectively (P <0.01). CONCLUSIONS: HCV infection in rural plasma was still chronic and occult. Most of the indicators of liver enzymology were mild to moderate abnormality. With the increase of HCV RNA load, the positive rate of anti-HCV and ALT Of the abnormal rate increased, so the three joint detection in the correct diagnosis and prediction of their liver damage and early treatment of great significance.