本实验观察了不同剂量LPS诱导大鼠肝Kupffer细胞释放TNF的作用。加入LPS后1小时,三种剂量LPS组Kupffer细胞培养上清中均可测到TNF活性,3小时达到峰值。100和150ng/ml LPS组TNF活性高于50ng/ml组(P<0.01),而100和150ng/ml两组之间无明显差异(P>0.05),再次加入LPS(终浓度100ng/ml),只有50ng/ml LPS组培养上清液中有TNF活性检出,但幅度明显下降(P<0.01)。上述结果提示LPS在体外诱导肝Kupffer细胞释放TNF在一定范围内具有剂量依赖关系,且呈一定的时间反应性。 In this experiment, we observed the effect of different doses of LPS on the release of TNF in rat liver Kupffer cells. One hour after the addition of LPS, TNF activity was detected in the supernatant of Kupffer cells in three doses of LPS group, reaching the peak at 3 hours. The TNF activity in 100 and 150ng / ml LPS group was higher than that in 50ng / ml group (P <0.01), while there was no significant difference between 100 and 150ng / ml group (P> 0.05) . Only TNF-α was detected in the culture supernatant of 50ng / ml LPS group, but the amplitude was significantly decreased (P <0.01). The above results suggest that LPS induced a dose-dependent manner in a certain range of TNF release induced by Kupffer cells in vitro, and showed a certain degree of temporal reactivity.